A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits.
Southeast Asian J Trop Med Public Health
;
2005 Mar; 36(2): 445-50
Artigo
em Inglês
| IMSEAR
| ID: sea-35863
ABSTRACT
A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits was compared with a conventional ELISA. This whole-blood ELISA has advantages. The volume of crude egg antigens, whole blood samples, and conjugates was only 0.05 ml. The incubation time was shortened to 5 minutes. Wells were washed three to five times with PBS-Tween after each procedure. Optical density values were measured in 10 minutes after transfer of 0.1 ml of substrate. Constant temperature was not necessary. The entire procedure took only 20-30 minutes.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Coelhos
/
Schistosoma japonicum
/
Esquistossomose
/
Fatores de Tempo
/
Ensaio de Imunoadsorção Enzimática
/
Programas de Rastreamento
/
Animais
/
Antígenos de Helmintos
Tipo de estudo:
Estudos de avaliação
/
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS