A preliminary analysis of Wuchereria bancrofti microfilarial antigens for potential use in diagnosis.
Southeast Asian J Trop Med Public Health
;
2000 Jun; 31(2): 252-8
Artigo
em Inglês
| IMSEAR
| ID: sea-35940
ABSTRACT
Several antigens from the microfilarial stage of Wuchereria bancrofti have been identified using immunoblots of microfilarial antigens and screening with immune sera and tropical pulmonary eosinophilia (TPE) sera. This analysis revealed an array of antigens with apparent molecular weights of 14kDa, 35kDa, 42kDa, 63kDa, 88kDa, 97kDa and 200kDa. Among these only the 14kDa and 42kDa antigens were consistently recognized by most of the immune sera. A 132kDa antigen was recognized only by TPE sera. Analysis of rabbit immune sera revealed that the 42kDa antigen was shared by two developmental stages of W. bancrofti, namely L3 and mF. This antigen could become a potential vaccine candidate. The 14kDa antigen seems specific for the microfilarial stage and therefore could be a diagnostic marker for active infection. The 132kDa antigen could aid in the diagnosis of TPE.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Eosinofilia Pulmonar
/
Coelhos
/
Wuchereria bancrofti
/
Humanos
/
Anticorpos Anti-Helmínticos
/
Vacinas
/
Immunoblotting
/
Imunofluorescência
/
Imunização
/
Reações Cruzadas
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2000
Tipo de documento:
Artigo
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