Antibiotic resistance, plasmid profile and RAPD-PCR analysis of enteropathogenic Escherichia coli (EPEC) clinical isolates.
Southeast Asian J Trop Med Public Health
;
2003 Sep; 34(3): 620-6
Artigo
em Inglês
| IMSEAR
| ID: sea-35942
ABSTRACT
Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhea among infants in developing countries. A total of 38 EPEC isolates, obtained from diarrhea patients of Hospital Miri, Sarawak, were investigated through plasmid profile, antibiotic resistance and randomly amplified polymorphic DNA (RAPD) analysis. From the 8 types of antibiotics used, all isolates were 100% resistant to furoxime, cephalothin and sulphamethoxazole and showed high multiple antibiotic resistant (MAR) indexes, ranging from 0.5 to 1.0. In plasmid profiling, 22 isolates (58%) showed the presence of one or more plasmids in the range 1.0 to 30.9 mDa. The dendrogram obtained from the results of the RAPD-PCR discriminated the isolates into 30 single isolates and 3 clusters at the level of 40% similarity. The EPEC isolates were highly diverse, as shown by their differing plasmid profiles, antibiotic resistance patterns and RAPD profiles.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmídeos
/
Humanos
/
Resistência a Medicamentos
/
Testes de Sensibilidade Microbiana
/
Valor Preditivo dos Testes
/
Impressões Digitais de DNA
/
Técnica de Amplificação ao Acaso de DNA Polimórfico
/
Adulto
/
Diarreia
/
Escherichia coli
Tipo de estudo:
Estudos de avaliação
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2003
Tipo de documento:
Artigo
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