Survival and infectivity of Brugia malayi microfilariae after cryopreservation.
Southeast Asian J Trop Med Public Health
;
1991 Jun; 22(2): 165-7
Artigo
em Inglês
| IMSEAR
| ID: sea-36165
ABSTRACT
Methods were studied for the cryopreservation of microfilariae of periodic Brugia malayi. RPMI-1640 tissue culture medium containing 6% dimethyl sulfoxide (DMSO) and 15% newborn calf serum was used as cryoprotectant. Samples were frozen slowly in the vapor phase of liquid nitrogen prior to emersion in liquid nitrogen (-196 degrees C). The freezing rate was -0.5 to -1.0 degrees C per minute, microfilariae remained viable for as long as, 212 and 375 days, survival rates were 94 to 98% and they were infective to Aedes togoi mosquitos. The infective larvae (L3) were obtained for 10-11 days after feeding at 28 degrees C room-temperature and the infection rate of L3 in test mosquitos was 22.4-30.6%. All DMSO should be removed from the freezing medium to restore microfilariae activity after freezing.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Brugia
/
Humanos
/
Distribuição de Qui-Quadrado
/
Criopreservação
/
Dimetil Sulfóxido
/
Aedes
/
Animais
/
Microfilárias
/
Nitrogênio
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1991
Tipo de documento:
Artigo
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