Determination of insecticide susceptibility in Culex quinquefasciatus Say adults by rapid enzyme microassays.
Southeast Asian J Trop Med Public Health
;
1992 Sep; 23(3): 458-63
Artigo
em Inglês
| IMSEAR
| ID: sea-36199
ABSTRACT
Rapid enzyme microassays for the detection of resistance due to organophosphate and carbamate in individual field-collected strains of Culex quinquefasciatus adults were conducted. These tests allowed accurate differentiation by eye, on the basis of color changes of susceptible and resistant individuals. Two separate tests were conducted for the biochemical assays. In the insensitive acetylcholinesterase (AChE) test, acetylthiocholine iodide (ACTH) and 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) were used as substrate and coupling agent respectively. The resulting yellow chromophore indicated AChE activity. Test results showed that the color intensity decreased as increasing concentrations of propoxur were added, thereby confirming the susceptibility of the enzyme to inhibitor. Assay of non-specific esterase however, indicated elevated levels which were correlated with degree of malathion resistance. Electrophoretic data revealed the presence of 2 esterase bands in all strains. It was concluded that such a pattern was not contributory to malathion resistance in adults.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Propoxur
/
Acetilcolinesterase
/
Fatores de Tempo
/
Bioensaio
/
Feminino
/
Resistência a Inseticidas
/
Culex
/
Relação Dose-Resposta a Droga
/
Eletroforese em Gel de Amido
/
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1992
Tipo de documento:
Artigo
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