Molecular epidemiology of malaria in endemic areas of Iran.
Southeast Asian J Trop Med Public Health
;
2003 ; 34 Suppl 2(): 15-9
Artigo
em Inglês
| IMSEAR
| ID: sea-36349
ABSTRACT
In this study, 333 blood samples of malaria cases positive by microscopic test (70.6% male and 29.4% female, p<0.05) were investigated. The group included 55 cases (16.52%) from Minab (Hormozgan Province), 116 cases (34.82%) from Iranshahr (Sistan-Baluchesta Province) and 162 cases (48.65%) from Kahnouj (Kerman Province). The results showed 244 cases (73.27%) were diagnosed as P. vivax, 87 cases (26.13%) P. falciparum and 2 cases (0.6%) showed a mixed infection of both Plasmodia. In a molecular study of the same samples using nested-polymerase chain reaction (nested-PCR), 185 cases (55.6%) were P. vivax, 50 cases (15%) P. falciparum and 95 cases (29%) both Plasmodia. Comparing the two methods used in this study, the highest rate of infection was found to be P. vivax. However, the rate of mixed infections (0.6% microscopy, 29% nested-PCR) varied and depended on the assay used. This indicated that the sensitivity of nested-PCR was greater than microscopic examination, especially for the detection of mixed-infections (p<0.05) in the current malaria epidemiology study.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Estações do Ano
/
Fatores Socioeconômicos
/
Feminino
/
Humanos
/
Masculino
/
Recém-Nascido
/
Criança
/
Pré-Escolar
/
Reação em Cadeia da Polimerase
/
Adolescente
Tipo de estudo:
Estudo de rastreamento
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2003
Tipo de documento:
Artigo
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