Large scale culture technique for pure Plasmodium falciparum gametocytes.
Southeast Asian J Trop Med Public Health
;
1997 Mar; 28(1): 18-21
Artigo
em Inglês
| IMSEAR
| ID: sea-36371
ABSTRACT
A large scale technique for pure gametocyte cultures of Plasmodium falciparum was established in culture flasks by using treated erythrocytes and RPMI medium supplemented with 15% human plasma instead of serum. Pure gametocyte cultures were successfully obtained following treatment with 5% sorbitol on day 8 and 9 of cultivation. This method resulted in approximately 97% reduction of asexual parasites and provided pure gametocytes in culture. The highest numbers of gametocytes were obtained from cultures starting with 2% parasitemia and 2% erythrocyte suspension. On day 12 of cultivation, approximately 35 x 10(6) gametocytes per 100 ml of cell suspension could be harvested.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmodium falciparum
/
Sorbitol
/
Feminino
/
Humanos
/
Masculino
/
Malária Falciparum
/
Meios de Cultivo Condicionados
/
Parasitemia
/
Eritrócitos
/
Gametogênese
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1997
Tipo de documento:
Artigo
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