Antigenic components of somatic extract from adult Fasciola gigantica recognized by infected human sera.
Asian Pac J Allergy Immunol
;
1997 Dec; 15(4): 213-8
Artigo
em Inglês
| IMSEAR
| ID: sea-36567
ABSTRACT
The antigenic components of Fasciola gigantica somatic extract were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technique using sera from patients with F. gigantica infection, patients with clinically diagnosed fascioliasis, patients with other infections/illness and healthy adults. By SDS-PAGE, it was found that the somatic product comprised more than 22 polypeptides. Immunoblotting analysis revealed at least 13 components which were strongly recognized by sera of patients with fascioliasis. These antigenic components had molecular weights ranging from less than 14.4 to more than 94 kDa. One antigenic component, i.e. 38 kDa was found to give a consistent reaction with sera of patients with fascioliasis (100% sensitivity and 96.7% specificity). The finding suggests that the 38 kDa components may be a potential diagnostic antigen for fascioliasis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Anticorpos Anti-Helmínticos
/
Adulto
/
Eletroforese em Gel de Poliacrilamida
/
Fasciolíase
/
Antígenos de Helmintos
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
1997
Tipo de documento:
Artigo
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