Flow cytometry for the analysis of T cells expressing CD69 after stimulation with glutamic acid decarboxylase.
Asian Pac J Allergy Immunol
;
2002 Mar; 20(1): 37-42
Artigo
em Inglês
| IMSEAR
| ID: sea-36603
ABSTRACT
Type 1 diabetes mellitus is a T-cell mediated autoimmune disease in which the insulin-producing pancreatic beta cells are selectively destroyed. We recently found that the detection of cell-mediated immune response to glutamic acid decarboxylase (GAD) was more useful than the detection of specific autoantibodies for the diagnosis of type 1 diabetes mellitus. In this study, we established a flow cytometric analysis for the detection of activated T cells in whole venous blood, obtained from diabetic patients and normal controls after stimulation by GAD. Two millitiers of peripheral venous blood and 6 hours incubation time were used for performing the test. It was found that 33% (3/9) type 1 diabetic patients, 7.7% (1/13) type 2 diabetic patients and neither patients with fibrocalculous pancreatopathy nor normal controls had > or = 20% CD8+ T cells expressing CD69. The results suggest that flow cytometry may be a useful tool for the detection of surrogate markers of type 1 diabetes mellitus.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tailândia
/
Idoso
/
Feminino
/
Humanos
/
Masculino
/
Ativação Linfocitária
/
Linfócitos T
/
Antígenos de Diferenciação de Linfócitos T
/
Antígenos CD
/
Adolescente
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
2002
Tipo de documento:
Artigo
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