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Distinct immunologic properties of Penicillium marneffei yeasts obtained from different in vitro growth conditions.
Asian Pac J Allergy Immunol ; 2004 Dec; 22(4): 229-35
Artigo em Inglês | IMSEAR | ID: sea-36643
ABSTRACT
A dimorphic fungus Penicillium marneffei is a causative agent of penicilliosis, a life-threatening disseminated disease in immunocompromised hosts predominantly found in southeast Asia and southern China. P. marneffei is the only known Penicillium that possesses a dimorphic characteristic. Since it is difficult to produce large amount of P. marneffei yeasts in vivo for experimentation purpose, yeast cells were produced in different in vitro conditions as alternatives. We interested in investigating the immunologic properties of yeast cells from different culture preparations. It was found that yeast cells obtained from brain heart infusion broth and Sabouraud dextrose broth did not resemble those resided in clinical specimens. A solution of 1% peptone, on the other hand, could induce a direct conidial transition into fission yeasts. Ability of yeast cells in each preparation to activate macrophages was determined by analyzing surface expression of CD40 and CD86 co-stimulatory molecules after two days of co-cultivation. Every P. marneffei yeast cell preparation demonstrated such ability. However, the ones from Sabouraud dextrose broth seemed to induce less phagocytosis. Additionally, although distinct antigenic profiles and lack of conformity in antigenic expression were observed among yeast cells from different culture conditions, most major immunogenic bands were present when Western analysis was performed using polyclonal antisera from penicilliosis patients. The results of the study raise attention on immunological and biochemical characteristics of P. marneffei yeasts if such preparations are to be used in future laboratory investigations.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Penicillium / Fagocitose / Leveduras / Glicoproteínas de Membrana / Antígenos CD / Regulação Fúngica da Expressão Gênica / Western Blotting / Antígenos CD40 / Meios de Cultura / Antígeno B7-2 Idioma: Inglês Revista: Asian Pac J Allergy Immunol Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Penicillium / Fagocitose / Leveduras / Glicoproteínas de Membrana / Antígenos CD / Regulação Fúngica da Expressão Gênica / Western Blotting / Antígenos CD40 / Meios de Cultura / Antígeno B7-2 Idioma: Inglês Revista: Asian Pac J Allergy Immunol Ano de publicação: 2004 Tipo de documento: Artigo