Monoclonal antibodies against protein antigens of salmonellae causing paratyphoid fever and their diagnostic application.
Asian Pac J Allergy Immunol
;
1995 Jun; 13(1): 63-70
Artigo
em Inglês
| IMSEAR
| ID: sea-36667
ABSTRACT
Hybrid clones producing monoclonal antibodies (MAbs) specific for Salmonella paratyphi A (72 clones), S. paratyphi B (9 clones) and S. paratyphi C (8 clones) were produced by using the affinity purified Salmonella protein (Bp) as immunogens. MAbs to S. paratyphi A and S. paratyphi B reacted specifically with the 52 kDa homologous flagellin protein components while those to S. paratyphi C reacted with a 61 kDa flagellin protein component. The MAbs against S. paratyphi A and S. paratyphi B were used to establish a double antibody sandwich ELISA for detection of the 52 kDa flagellin antigens in serum samples from patients with acute paratyphoid A and paratyphoid B fever. With this assay system, 6.25 ng per ml of flagellin antigens of S. paratyphi A and S. paratyphi B could be detected. However, the assay system could not detect the flagellin antigens in patients' sera. The presence of IgM antibodies to the 52 kDa antigens of S. paratyphi A and S. paratyphi B in the acute sera from paratyphoid A or paratyphoid B patients suggested that the 52 kDa protein components of both salmonellae are good immunogens for human and might be used as antigens for early diagnosis of paratyphoid A and paratyphoid B fever.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Salmonella
/
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Febre Paratifoide
/
Flagelina
/
Anticorpos Monoclonais
/
Antígenos de Bactérias
Tipo de estudo:
Estudo de rastreamento
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
1995
Tipo de documento:
Artigo
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