Isolation and characterization of allergens from the seeds of Vigna sinensis.
Asian Pac J Allergy Immunol
;
2000 Mar; 18(1): 9-14
Artigo
em Inglês
| IMSEAR
| ID: sea-36701
ABSTRACT
Allergenic components of cowpea vegetable green seeds (Vigna sinensis) were isolated based on solubility, isoelectric precipitation and molecular mass. The allergenicity of the cowpea fractions was monitored by enzyme-linked immunosorbent assay (ELISA) and skin-prick test. The allergenic albumin fraction was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-specific immunoblotting. The 41 and 55 kDa protein components were found to be major allergens and the allergenicity was resistant to heat and proteolytic enzyme digestion. This study confirms the presence of potent allergens in cowpea seeds.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Sementes
/
Proteínas de Vegetais Comestíveis
/
Humanos
/
Imunoglobulina E
/
Ensaio de Imunoadsorção Enzimática
/
Alérgenos
/
Immunoblotting
/
Criança
/
Cromatografia em Gel
/
Adolescente
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
2000
Tipo de documento:
Artigo
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