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Detection and differentiation of human herpesviruses 1-5 by consensus primer PCR and RFLP.
Asian Pac J Allergy Immunol ; 2003 Mar; 21(1): 55-61
Artigo em Inglês | IMSEAR | ID: sea-36800
ABSTRACT
Eight human viruses of the Herpesviridae family represent a significant public health problem world-wide. Detection and typing of five of the human herpesviruses (HSV-1, HSV-2, VZV, EBV, and CMV) was performed by applying a consensus primer polymerase chain reaction (PCR). The amplified PCR products from the five human herpesviruses were typed based on their restriction enzyme digestion polymorphism with Hinf I and Alu I. Fifteen clinically suspected specimens from herpesvirus-infected patients were also evaluated. A fragment of the DNA polymerase gene from each of the five human herpesviruses was successfully amplified by the set of consensus primers. Their amplicons obtained by PCR from the template DNAs were subjected to restriction endonuclease digestion and human herpesviruses 1-5 could be clearly differentiated and typed. This method can be used to detect and differentiate between the five human herpesviruses in clinical specimens. This study demonstrates the value of testing for five human herpesviruses by consensus PCR and restricted fragment length polymorphism (RFLP). These procedures are simple and straightforward techniques for the investigation of clinical specimens.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Polimorfismo de Fragmento de Restrição / Humanos / DNA Viral / Sequência de Bases / Criança / Reação em Cadeia da Polimerase / Herpesviridae Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Asian Pac J Allergy Immunol Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Polimorfismo de Fragmento de Restrição / Humanos / DNA Viral / Sequência de Bases / Criança / Reação em Cadeia da Polimerase / Herpesviridae Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Asian Pac J Allergy Immunol Ano de publicação: 2003 Tipo de documento: Artigo