Identification of circulating antibodies in fasciolosis and localization of 66 kDa antigenic target using monoclonal antibodies.
Asian Pac J Allergy Immunol
;
1999 Mar; 17(1): 53-8
Artigo
em Inglês
| IMSEAR
| ID: sea-37053
ABSTRACT
We identified three specific circulating antibodies in serum of cattle naturally infected with Fasciola gigantica. Two of the antibodies were found to react specifically to 97 and 66 kDa antigenic molecules of adult worm tegumental membrane extract. The third antibody was identified by the reaction with 26-28 kDa molecule of the excretory/secretory antigens. Monoclonal antibody against 66 kDa protein was developed and used for localization of its antigenic target in adult worm frozen sections. The experiment demonstrated that 66 kDa protein is a component on the outer surface membrane and on the membrane lining of the caecal epithelial of adult worm. The 66 kDa antigen was considered as a promising candidate for immunodiagnosis and vaccine.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Bovinos
/
Anticorpos Anti-Helmínticos
/
Microscopia Eletrônica de Varredura
/
Immunoblotting
/
Doenças dos Bovinos
/
Octoxinol
/
Fasciola
/
Fasciolíase
/
Animais
/
Anticorpos Monoclonais
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
1999
Tipo de documento:
Artigo
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