Comparative study of respiratory syncytial virus in nasopharyngeal aspirates using conventional cell culture, shell viral centrifugation culture, immunofluorescence and biotin-avidin enzyme linked immunosorbent assays.
Asian Pac J Allergy Immunol
;
1991 Dec; 9(2): 121-4
Artigo
em Inglês
| IMSEAR
| ID: sea-37056
ABSTRACT
133 nasopharyngeal aspirates (NPA) were simultaneously tested for the presence of respiratory syncytial virus (RSV) by conventional cell culture (CCC), shell vial centrifugation culture (SVC), immunofluorescence assay (IFA) and biotin-avidin enzyme linked immunosorbent assay (B-A ELISA). These yielded positive results in 32(24%), 45(33.8%), 36(27%) and 40(30%) of specimens, respectively. Specimens positive by IFA and B-A ELISA were all also positive by SVC. The sensitivity of CCC, IFA, and B-A ELISA comparing to SVC was 71%, 80%, and 88.9%, respectively. For rapid detection of RSV, we recommend the SVC method where a cell culture laboratory is available and the B-A ELISA method where a cell culture laboratory is not available.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Infecções por Respirovirus
/
Vírus Sinciciais Respiratórios
/
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Centrifugação
/
Nasofaringe
/
Imunofluorescência
/
Sensibilidade e Especificidade
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
1991
Tipo de documento:
Artigo
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