Comparison between dual and tri-colour reagents for the analysis of lymphocyte subsets.
Asian Pac J Allergy Immunol
;
1996 Dec; 14(2): 87-90
Artigo
em Inglês
| IMSEAR
| ID: sea-37096
ABSTRACT
The aim of this project was to compare dual and tri-colour reagents for lymphocyte immunophenotyping. A total of 37 patient and normal specimens were immunophenotyped concurrently with the following mean values (% dual vs tri-colour) CD3 (69.4 vs 68.3) CD4 (24.0 vs 24.2) and CD19 (13.9 vs 12.6). A comparison of the results obtained using the paired t test showed that there were no significant differences for cells expressing CD3, CD4 and CD19. However, there was a significant difference in the NK (18.3 vs 16.3) cell component. A major advantage in using 3 colour immunophenotyping is the ability to analyse specimens that cannot be analysed using dual colour reagents due to debris or contamination of the gate with non-lymphocytic cells.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Células Matadoras Naturais
/
Antígenos HLA-DR
/
Antígenos CD4
/
Subpopulações de Linfócitos
/
Complexo CD3
/
Antígenos CD19
/
Corantes
/
Citometria de Fluxo
/
Indicadores e Reagentes
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
1996
Tipo de documento:
Artigo
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