Antinuclear antibody detection using streptavidin-biotin-peroxidase complex on HEp-2 cell substrate.
Asian Pac J Allergy Immunol
;
1992 Jun; 10(1): 19-24
Artigo
em Inglês
| IMSEAR
| ID: sea-37242
ABSTRACT
The streptavidin-biotin-peroxidase complex (SABC) technique was compared to conventional indirect immunofluorescence (IIF) for the detection of anti-nuclear antibody (ANA) on HEp-2 cell substrate. SABC showed higher specificity and predictive value and gave more reproducible titres and clearer staining patterns than IIF in sera from a series of rheumatic disease patients. Sera from 80 patients with various types of rheumatic diseases and 20 without rheumatic disease were further tested using the SABC method. All systemic lupus erythematosus (SLE) sera were positive. The overall sensitivity was 95%, specificity 90% and predictive value 97% for rheumatic disease. The rim pattern was associated with SLE and mixed connective tissue disease. The nucleolar/homogeneous pattern was associated with scleroderma and SLE in remission. ANA titre and staining pattern have limited value in the clinical assessment of rheumatic disease; however, ANA has very high sensitivity for SLE and remains an excellent screening test.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Prognóstico
/
Humanos
/
Células Tumorais Cultivadas
/
Anticorpos Antinucleares
/
Doenças Reumáticas
/
Valor Preditivo dos Testes
/
Imunofluorescência
/
Técnicas Imunoenzimáticas
/
Sensibilidade e Especificidade
/
Lúpus Eritematoso Sistêmico
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Asian Pac J Allergy Immunol
Ano de publicação:
1992
Tipo de documento:
Artigo
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