Acridine orange staining and viability of the coccoid form of Campylobacter upsaliensis.
Artigo
em Inglês
| IMSEAR
| ID: sea-38369
ABSTRACT
Conversion of Campylobacter upsaliensis to the coccoid form during aerobic incubation at 37 degrees C was not prevented by treatment with chloramphenicol and was accompanied by severe decreases in isocitrate dehydrogenase activity and oxygen uptake. Although the coccoid forms fluoresced orange-red by acridine orange staining, agarose gel electrophoresis indicated an extensive degradation of the ribosomal RNA. This suggests that acridine orange staining may not be a good indicator of viability and that the coccoid form of C. upsaliensis at 37 degrees C is degenerative rather than part of the life cycle.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Consumo de Oxigênio
/
Laranja de Acridina
/
RNA Ribossômico
/
Campylobacter upsaliensis
/
Viabilidade Microbiana
/
Corantes Fluorescentes
/
Isocitrato Desidrogenase
Idioma:
Inglês
Ano de publicação:
2005
Tipo de documento:
Artigo
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