BCR/ABL rearrangement in Philadelphia chromosome negative CML patients.
Artigo
em Inglês
| IMSEAR
| ID: sea-39275
ABSTRACT
Reverse transcription-polymerase chain reaction (RT-PCR) was used to study 34 patients with chronic myelogenous leukemia (CML) associated with negative Philadelphia (Ph) chromosome. This report showed evidence of a chimeric BCR/ABL transcript in 18 (52.9%) and 28 (82.4%) cases by first PCR and seminested PCR, respectively. In these BCR/ABL transcript positive cases, the incidence of BCR exon3/ABL exon2 (B3A2) and BCR exon 2/ABL exon2 rearrangement was 25 (89.3%) and 3 (10.7%) cases, respectively. The other 6 Ph negative patients showed no evidence of reciprocal translocation of BCR to chromosome 9. This data demonstrates that seminested PCR is sufficiently sensitive to detect BCR/ABL fusion transcript in Ph chromosome negative CML patients.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Valores de Referência
/
Cromossomo Filadélfia
/
Feminino
/
Humanos
/
Masculino
/
Leucemia Mielogênica Crônica BCR-ABL Positiva
/
Amplificação de Genes
/
Proteínas de Fusão bcr-abl
/
Sensibilidade e Especificidade
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Ano de publicação:
2000
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS