Prenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling.
Artigo
em Inglês
| IMSEAR
| ID: sea-39403
ABSTRACT
OBJECTIVE:
To describe the experience of prenatal diagnosis for Hb Bart's disease, by chorionic villus sampling (CVS) with DNA analysis.DESIGN:
Descriptive study SETTINGS Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University.SUBJECTS:
Sixteen high risk pregnancies at risk of Hb Bart's disease who were eligible for CVS criteria between 1 January, 1999 and May 31, 2000. MATERIAL ANDMETHOD:
Fetal villi were obtained by either transcervical (TC) or transabdominal (TA) CVS route to extract DNA and detect for alpha-thal-1 gene deletion (SEA type) with modified Chang's method. The CVS results were confirmed by either serial ultrasound or cordocentesis or diagnosis after pregnancy termination. MAIN OUTCOMEMEASURES:
The efficacy, safety and pregnancy outcomes.RESULTS:
CVS was successfully done in all of 16 cases (5 with TC and 11 with TA), The mean gestational age was 13.25 +/- 2.9 weeks. The procedure time for TA was shorter than that of TC (4.64 +/- 5.4 vs 10.4 +/- 11.3 min). The CVS result showed as follows 3 normal fetuses, 7 alpha-thal-1 carriers, 4 fetal Hb Bart's, 1 misdiagnosis and 1 failure to diagnosis due to technical error. The sensitivity and specificity were 100 per cent (4/4) and 90.91 per cent (10/11), respectively. One case of Hb Bart's misdiagnosis and one failure case were later confirmed for alpha-thal-1 trait and alpha-thal-1/ Hb E trait by cordocentesis, respectively. The pregnancy outcomes included 11 livebirths, 4 terminated cases and 1 fetal loss of continuing pregnancies. No serious complications occurred.CONCLUSION:
This preliminary experience suggests that CVS is an effective method for early prenatal diagnosis of fetal Hb Bart's.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Feminino
/
Humanos
/
Gravidez
/
Resultado da Gravidez
/
Amostra da Vilosidade Coriônica
/
Reação em Cadeia da Polimerase
/
Talassemia alfa
/
Gravidez de Alto Risco
/
Doenças Fetais
Idioma:
Inglês
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS