Vitrification of mouse oocyte using open pulled straws compared with needles.
Artigo
em Inglês
| IMSEAR
| ID: sea-40522
ABSTRACT
OBJECTIVE:
To compare the survival rate of mouse oocytes and fertilization rate between using open pulled straws (OPS) and needles for vitrification. MATERIAL ANDMETHOD:
Meiosis II oocytes from female C57B/6J mice aged 7-8 weeks were collected and allocated to two groups for vitrification by using OPS or needles. Vitrified oocytes were thawed, morphological survival and fertilization rate were examined.RESULTS:
There was no obvious difference between the morphological survival rates of vitrified mouse oocytes using OPS and needles (66.7% vs 64.8%). Proportions Difference 1.9% (95% CI -7.1, 10.7). The vitrified oocytes from the needle had significantly higher percentages of fertilization rate than OPS (76.8% vs 62.5%). Proportions Difference -14.3% (95% CI -24.5, -3.6).CONCLUSION:
Vitrification method of mouse oocytes using needles when compared to OPS provides a similar morphological survival rate and higher fertilization rate.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oócitos
/
Feminino
/
Criopreservação
/
Animais
/
Meiose
/
Camundongos
/
Camundongos Endogâmicos C57BL
/
Agulhas
Idioma:
Inglês
Ano de publicação:
2006
Tipo de documento:
Artigo
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