A simple method for protein electrophoresis of unconcentrated urine.
Artigo
em Inglês
| IMSEAR
| ID: sea-42799
ABSTRACT
Thirty-two unconcentrated urines were electrophoresed on cellogels in 0.05 M barbital buffer, pH 8.6, at 280 volts for 35 minutes. Instead of concentrating 100 fold, the urine samples were directly applied on the gel in volumes from 1.2 to 3.6 microliters and Coomassie dye was used for staining. This makes it possible to observe the protein patterns at trace concentration by reagent strips (about 0.05-0.2 g/l). This method is simple, convenient and suited for routine services.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteinúria
/
Humanos
/
Eletroforese
Idioma:
Inglês
Ano de publicação:
1990
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS