Feasibility study on the detection of ferritin using surface plasmon resonance.
Artigo
em Inglês
| IMSEAR
| ID: sea-43811
ABSTRACT
Determination of ferritin concentration was performed using surface plasmon resonance technique. An anti-human ferritin antiserum from rabbits was immobilized on the protein-A coated sensor chip. The resonance change due to the binding of ferritin to the antibody was measured. It was found that the antigen-antibody binding of the ferritin could be monitored in real time. The detection of ferritin could be obtained in the range of 25-800 ng/ml. This dynamic range could be applied in various medical applications. Moreover, it was observed that the sensor chips could be regenerated. This offers an opportunity for multiple uses, which will reduce the unit operating cost.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Estudos de Viabilidade
/
Sensibilidade e Especificidade
/
Ressonância de Plasmônio de Superfície
/
Deficiências Nutricionais
/
Ferritinas
/
Ferro
/
Reações Antígeno-Anticorpo
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Ano de publicação:
2001
Tipo de documento:
Artigo
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