Evaluation of a locally developed direct immunofluorescence test for chlamydial infections.
Artigo
em Inglês
| IMSEAR
| ID: sea-44632
ABSTRACT
The high cost of diagnostic tests for chlamydial infections limits their use which may result in under estimation of the incidence of chlamydial infections. This study was an attempt to reduce the cost of the test by developing an immunofluorescence test for C. trachomatis using monoclonal antibody to major outer membrane protein of C. trachomatis. Urethral swabs were obtained from patients with symptoms of urethritis. The developed immunofluorescence test was compared with culture method and a commercial immunofluorescence test kit (BioMerieux). Compared with the culture method, the sensitivity, specificity, predictive value of positive and predictive value of negative of the developed test were 79, 85, 61 and 93 per cent respectively. The results obtained from the comparison with commercial test kit showed an agreement of 88 per cent. The developed test was positive in 32 per cent of specimens while the commercial test was positive in 24 per cent. The commercial test kit showed excellent reactions and it contained monoclonal antibody to lipopolysaccharide of Chlamydiae in addition to monoclonal antibody to major outer membrane protein which can lead to stronger immunofluorescence staining. The locally developed test, however, costs much less without compromising the results.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Tailândia
/
Uretrite
/
Humanos
/
Masculino
/
Infecções por Chlamydia
/
Chlamydia trachomatis
/
Imunofluorescência
/
Sensibilidade e Especificidade
/
Estudos de Avaliação como Assunto
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo prognóstico
País/Região como assunto:
Ásia
Idioma:
Inglês
Ano de publicação:
1992
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS