Reverse line probe assay for the rapid detection of rifampicin resistance in Mycobacterium leprae.
Artigo
em Inglês
| IMSEAR
| ID: sea-46690
ABSTRACT
Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Rifampina
/
Bioensaio
/
Biópsia
/
Humanos
/
Reação em Cadeia da Polimerase
/
Farmacorresistência Bacteriana
/
Genes Bacterianos
/
Hansenostáticos
/
Hanseníase
/
Mycobacterium leprae
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Ano de publicação:
2006
Tipo de documento:
Artigo
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