Atypical rotavirus identified from young children with diarrhoea in South Africa.
J Health Popul Nutr
;
2001 Sep; 19(3): 199-203
Artigo
em Inglês
| IMSEAR
| ID: sea-527
ABSTRACT
The study was undertaken for antigenic characterization of rotavirus strains isolated from South African children. During July 1996-July 1997, an epidemiological surveillance of rotavirus-associated gastroenteritis was carried out in Pretoria and Gauteng provinces. In total, 1,229 diarrhoeal faecal samples were collected from a pathology laboratory. Of 389 (32%) rotavirus strains detected, most (97%) were group A rotaviruses by serological assay. However, 12 rotavirus strains identified did not apparently carry the common group A-specific antigen as determined by both commercial and monoclonal antibody-based ELISAs. Electrophoretic analysis of the viral RNA genome revealed that these strains shared a common electropherotype and that this resembled the group A rotavirus constellation of RNA segments. Furthermore, the conserved terminal sequences of the group A VP6 and VP7 genes could not be targeted by standard reverse transcriptase-polymerase chain reaction with a routine set of primers to the VP6 or VP7 genes. These strains present an interesting phenotypic variation of the recognized rotaviruses and warrant further characterization.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
África do Sul
/
Humanos
/
Recém-Nascido
/
RNA Viral
/
Pré-Escolar
/
Rotavirus
/
Adulto
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Lactente
/
Antígenos Virais
País/Região como assunto:
África
Idioma:
Inglês
Revista:
J Health Popul Nutr
Assunto da revista:
Gastroenterology
/
Nutritional Sciences
/
Public Health
Ano de publicação:
2001
Tipo de documento:
Artigo
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