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Aetiology of shigellosis in northern Pakistan.
J Health Popul Nutr ; 2003 Mar; 21(1): 32-9
Artigo em Inglês | IMSEAR | ID: sea-528
ABSTRACT
People of northern Pakistan face health hazards because of poor sanitation practices. Bacterial gastrointestinal infections are very common, and sometimes outbreaks occur. The present study was aimed at evaluating and analyzing infestation of Shigella spp. in patients with suspected gastroenteritis and ascertaining the status of antibiotic therapy. Five hundred and eighty-five faecal samples of patients with suspected gastroenteritis, referred to the District Headquarter Hospital Gilgit, were investigated for common enteropathogenic bacteria from July 1997 to September 1999. Seventy-seven (13.2%) of the faecal specimens were infected with different strains of Shigella spp., 61% of which were Shigella dysenteriae, 15.6% were S. flexneri, and 23.4% were Shigella sp. All Shigella strains were sensitive to ceftriaxone, cefotaxime, ciprofloxacin, and enoxacin. Sixty-one percent of the strains were resistant to both ampicillin and chloramphenicol, and 3.9% to ampicillin and nalidixic acid, while 10.4% were resistant to ampicillin alone and 14.3% to chloramphenicol only. Only 10.4% of the strains were sensitive to all the antibiotics tested. Sixty strains of Shigella spp. were processed for isolation of plasmids, and 58 (97%) of these antibiotic-resistant bacteria harboured at least one plasmid. The number of plasmids varied from 1 to 9. Escherichia coli C600 were transformed with the isolated plasmids. Transformants, containing 23-kb plasmid, resisted growth in media containing antibiotics, thereby indicating that antibiotic resistance is plasmid-borne. Based on the findings of the study, it is concluded that the infestation of Shigella spp. is high in northern Pakistan, the aetiological agents are highly resistant to chloramphenicol and ampicillin, and the antibiotic resistance is mediated by the 23-kb plasmid.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Paquistão / Plasmídeos / Transformação Bacteriana / Feminino / Humanos / Masculino / Criança / Adolescente / Distribuição por Sexo / Distribuição por Idade País/Região como assunto: Ásia Idioma: Inglês Revista: J Health Popul Nutr Assunto da revista: Gastroenterology / Nutritional Sciences / Public Health Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Paquistão / Plasmídeos / Transformação Bacteriana / Feminino / Humanos / Masculino / Criança / Adolescente / Distribuição por Sexo / Distribuição por Idade País/Região como assunto: Ásia Idioma: Inglês Revista: J Health Popul Nutr Assunto da revista: Gastroenterology / Nutritional Sciences / Public Health Ano de publicação: 2003 Tipo de documento: Artigo