Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization.
Indian J Med Microbiol
;
2007 Oct; 25(4): 391-4
Artigo
em Inglês
| IMSEAR
| ID: sea-53479
ABSTRACT
Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to detect as low as 10 3 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Pseudomonas
/
Infecções por Pseudomonas
/
Acinetobacter
/
Sangue
/
Infecções por Acinetobacter
/
Humanos
/
Técnicas Bacteriológicas
/
Sensibilidade e Especificidade
/
Bacteriemia
/
Hibridização in Situ Fluorescente
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Idioma:
Inglês
Revista:
Indian J Med Microbiol
Assunto da revista:
Microbiology
Ano de publicação:
2007
Tipo de documento:
Artigo
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