Multiplex PCR on leptospiral isolates from Kolenchery, Kerala, India.
Indian J Med Microbiol
;
2005 Apr; 23(2): 114-6
Artigo
em Inglês
| IMSEAR
| ID: sea-53700
ABSTRACT
Human leptospirosis causes severe multiorgan dysfunctions that may end in multiorgan failure and death. The methods in hand for diagnosis of leptospirosis like culture, ELISA and MAT only help to confirm the disease, and are of little value in early detection. The aim of this study was to find out if the two sets of primers described earlier could detect all the isolates from the area, for the purpose of using the resultant database for early detection of leptospires in future from clinical specimens. The study was done on culture isolates from Jan 2000 to June 2002 attending the department of medicine, MOSC medical college hospital, Kolenchery, Kerala, India. DNA of 45 culture isolates were amplified by multiplex PCR using two sets of previously described primers, G1, G2 and B 64-I, B 64-II. Specific amplifications of either 285 or 563 bp size were obtained from all isolates included in the study indicating the utility of the multiplex PCR in the rapid detection of leptospires in clinical samples.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Especificidade da Espécie
/
Humanos
/
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Hospitais de Condado
/
Índia
/
Leptospira
/
Leptospirose
Tipo de estudo:
Estudo de rastreamento
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Indian J Med Microbiol
Assunto da revista:
Microbiology
Ano de publicação:
2005
Tipo de documento:
Artigo
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