Advantage of using a home-made ELISA kit for detection of Helicobacter pylori infection over commercially imported kits.
Indian J Med Microbiol
;
2008 Apr-Jun; 26(2): 127-31
Artigo
em Inglês
| IMSEAR
| ID: sea-53770
ABSTRACT
PURPOSE:
To evaluate a home-made ELISA kit for detection of Helicobacter pylori (Hp) infection and comparison of its immunologic criteria with those of foreign commercial kits.METHODS:
A home-made IgG ELISA kit was developed using soluble antigenic fractions of Hp proteins. Confirmed sera were tested and serological criteria were evaluated through assessment of 199 serum samples.RESULTS:
The accuracy, sensitivity and specificity values of home-made kit were 92, 92 and 90.4%, respectively. These immunologic criteria for Trinity kit were 95.2, 95.2 and 95% in comparison with IBL kit (91.3, 92.2 and 88.5%), BIOHIT kit (72.4, 41.6 and 94.1%) and HelicoBlot2.1 (94.2, 93.4 and 100%). Kappa agreement assessment demonstrated that two of the imported ELISA kits had fair to moderate agreement with the home-made kit while the other one had a poor agreement value.CONCLUSIONS:
Apart from comparable values between the home-made kit and the most efficient imported kit (Trinity) there was significant cost benefit. Therefore, we recommend the home-made kit as a suitable substitution for detection of Hp infection in the Iranian population.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Idoso de 80 Anos ou mais
/
Idoso
/
Feminino
/
Humanos
/
Masculino
/
Ensaio de Imunoadsorção Enzimática
/
Helicobacter pylori
/
Infecções por Helicobacter
/
Sensibilidade e Especificidade
/
Adolescente
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Limite:
Aged80
Idioma:
Inglês
Revista:
Indian J Med Microbiol
Assunto da revista:
Microbiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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