Rapid antibiotic susceptibility testing of Mycobacterium tuberculosis: its utility in resource poor settings.
Indian J Med Microbiol
;
2006 Oct; 24(4): 268-72
Artigo
em Inglês
| IMSEAR
| ID: sea-53871
ABSTRACT
PURPOSE:
To compare the rapid colorimetric nitrate reductase based antibiotic susceptibility (CONRAS) test performed on Mycobacterium tuberculosis isolates with the conventional method i.e, the proportion method.METHODS:
One hundred clinical isolates of M. tuberculosis were tested for susceptibility to isoniazid (INH) and rifampicin (RIF) by the conventional proportion method and CONRAS in Middlebrook 7H9 liquid medium enriched with growth supplements (MB7H9S).RESULTS:
The performance of the CONRAS test was evaluated using proportion method as the gold standard. The sensitivity (ability to detect true drug resistance) and specificity (ability to detect true drug susceptibility) of the CONRAS test to INH was 93.75 and 98.52% and for RIF it was 96.10 and 100% respectively. The mean time for reporting was 6.3 days and the test showed excellent reproducibility. The kappa (k) value for INH was 0.92 and for RIF was 0.99, indicating excellent agreement between the two methods.CONCLUSIONS:
CONRAS test is a rapid and reliable method of drug susceptibility for M. tuberculosis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Pobreza
/
Rifampina
/
Fatores de Tempo
/
Tuberculose Pulmonar
/
Humanos
/
Testes de Sensibilidade Microbiana
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Tuberculose Resistente a Múltiplos Medicamentos
/
Colorimetria
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Idioma:
Inglês
Revista:
Indian J Med Microbiol
Assunto da revista:
Microbiology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS