Antiviral activity of the Indian medicinal plant extract Swertia chirata against herpes simplex viruses: a study by in-vitro and molecular approach.
Indian J Med Microbiol
;
2008 Oct-Dec; 26(4): 322-6
Artigo
em Inglês
| IMSEAR
| ID: sea-53872
ABSTRACT
PURPOSE:
The antiviral activity of Indian Medicinal plant extract Swertia chirata was tested against Herpes simplex virus (HSV) type-1, using multiple approaches both at cellular and molecular level.METHODS:
Cytotoxicity, plaque reduction, virus infectivity, antigen expression and polymerase chain reaction (PCR) assays were conducted to test the antiviral activity of the plant extract.RESULTS:
Swertia plant crude extract (1 gm/mL) at 164 dilution inhibited HSV-1, plaque formation at more than 70% level. HSV antigen expression and time kinetics experiments conducted by indirect immunofluorescence (IFA) test, revealed a characteristic pattern of small foci of single fluorescent cells in Swertia extract treated HSV-1 infected cells at 4 hours post infection dose, suggested drug inhibited viral dissemination. Infected cell cultures treated with Swertia extract at various time intervals, tested by PCR, failed to show amplification at 12, 24-72 hours. HSV-1 infected cells treated with Acyclovir (antiviral drug) did not show any amplification by PCR.CONCLUSIONS:
In this preliminary study, the Indian medicinal plant extract, Swertia chirata showed antiviral properties against Herpes simplex virus type-1.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Antivirais
/
Plantas Medicinais
/
Ensaio de Placa Viral
/
Células Vero
/
Humanos
/
Aciclovir
/
Extratos Vegetais
/
Chlorocebus aethiops
/
Reação em Cadeia da Polimerase
/
Herpesvirus Humano 1
Idioma:
Inglês
Revista:
Indian J Med Microbiol
Assunto da revista:
Microbiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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