Comparative evaluation of enzyme immunoassays based on synthetic glycoconjugates and phenolic glycolipid-I for immunodiagnosis of leprosy.
Indian J Lepr
;
1990 Jan-Mar; 62(1): 60-5
Artigo
em Inglês
| IMSEAR
| ID: sea-54277
ABSTRACT
Enzyme immunoassays (EIAs) based on synthetic glycoconjugates containing the terminal monosaccharide (M-BGG) or disaccharide (ND-BSA) residue of the trisaccharide component of phenolic glycolipid-I (PGL-I), for immunodiagnosis of leprosy are described. The results of the assays were compared with that of the EIA using PGL-I. All the three assays were highly specific for leprosy. The per cent positivity of active lepromatous leprosy (LL) patients with M-BGG was 78.05 in comparison to 85.36 with ND-BSA and 82.11 with PGL-I. Similarly, the positivity of tuberculoid (TT) leprosy patients in M-BGG assay was lower than that in EIAs using ND-BSA or PGL-I. However, the difference in the positivity of individual category of leprosy patients in the three EIAs was not statistically significant. The correlation between absorbance values of leprosy sera in EIAs based on M-BGG and PGL-I, as well as that in assays using ND-BSA and PGL-I was statistically significant.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Imunoglobulina M
/
Glicoconjugados
/
Glicolipídeos
/
Técnicas Imunoenzimáticas
/
Hanseníase
/
Mycobacterium leprae
/
Antígenos de Bactérias
Idioma:
Inglês
Revista:
Indian J Lepr
Assunto da revista:
Tropical Medicine
Ano de publicação:
1990
Tipo de documento:
Artigo
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