Certain aspects of dapsone metabolism in leprosy patients as studied by high performance liquid chromatography (HPLC) and qualitative screening tests.
Indian J Lepr
;
1988 Apr; 60(2): 215-24
Artigo
em Inglês
| IMSEAR
| ID: sea-54536
ABSTRACT
Dapsone (DDS) in urine of 250 leprosy patients collected on surprise visits were screened by simple paper spot, tile tests and sensitive Enzyme linked immunosorbent assay (ELISA) and Haemagglutination inhibition (HI) tests. The urinary DDS concentration as well as DDS/C ratios were also studied. Simultaneously, 50 microliter of blood was collected from each of these patients and its dapsone content was estimated by HPLC. Urine samples with means of 25 to 30 micrograms/ml DDS and 55-64 micrograms/mg DDS/C ratios were found to give positive tests by any of the above screening procedures, while their mean blood DDS concentration was found to be 0.91 microgram/ml. The corresponding values for those specimens giving negative tests were 3.8 to 5.7 micrograms DDS per ml and 9 to 13 micrograms/mg DDS/C ratio. The blood DDS concentration in this group was ranging from 0.16 to 0.18 micrograms/ml. The findings are discussed in relation to their metabolic significance and their application in a leprosy control programme.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Testes de Inibição da Hemaglutinação
/
Taxa de Depuração Metabólica
/
Valor Preditivo dos Testes
/
Cromatografia Líquida de Alta Pressão
/
Cooperação do Paciente
/
Dapsona
/
Hanseníase
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Pesquisa qualitativa
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Indian J Lepr
Assunto da revista:
Tropical Medicine
Ano de publicação:
1988
Tipo de documento:
Artigo
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