An in vitro culture method for screening new drugs against Mycobacterium leprae.
Indian J Lepr
;
1990 Jan-Mar; 62(1): 66-75
Artigo
em Inglês
| IMSEAR
| ID: sea-54756
ABSTRACT
An in vitro culture system has been devised for the maintenance and growth of M. leprae in a cell-free medium. Cells from four-week old cultures could be transferred to fresh medium and normal growth was observed in subcultures. Using this system, the M.I.Cs of dapsone and rifampicin were determined. Dapsone at 25 ng/ml and rifampicin at 300 ng/ml completely inhibited the growth of host-grown as well as in vitro-adapted M. leprae. It was further shown that the effects of both the drugs were bactericidal; this observation was subsequently confirmed using mouse foot-pad technique.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Rifampina
/
Meios de Cultura
/
Dapsona
/
Animais
/
Mycobacterium leprae
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Indian J Lepr
Assunto da revista:
Tropical Medicine
Ano de publicação:
1990
Tipo de documento:
Artigo
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