Detection of Pasteurella multocida in experimentally infected embryonated chicken eggs by PCR assay.
Indian J Exp Biol
;
2006 Apr; 44(4): 321-4
Artigo
em Inglês
| IMSEAR
| ID: sea-55946
ABSTRACT
Applicability of polymerase chain reaction (PCR) assay to detect Pasteurella multocida in experimentally infected embryonated chicken egg was assessed in the present study. PCR assay rapidly and specifically detected the genome of P. multocida in amniotic fluid, allantoic fluid and homogenates of infected embryo and its membranes. The sensitivity of detection was as low as 20 bacterial cells/ml of allantoic or amniotic fluids. Detection of P. multocida in dead embryos by PCR was possible up to 6 and 30 days or more following storage of dead embryos at 37 degrees C, and at 4 degrees C as well as at -20 degrees C, respectively. The study revealed that PCR assays could be employed directly for detection and confirmation of P. multocida infection in experimentally infected chicken embryos.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Infecções por Pasteurella
/
Temperatura
/
Fatores de Tempo
/
Embrião de Galinha
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
Pasteurella multocida
/
Animais
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2006
Tipo de documento:
Artigo
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