DNA isolation from goat blood using different brands of household detergents and its downstream application.
Indian J Exp Biol
;
2006 Oct; 44(10): 852-4
Artigo
em Inglês
| IMSEAR
| ID: sea-56007
ABSTRACT
Rapid isolation of DNA from goat blood using different brands of detergents available in Indian market, is reported. The integrity and efficiency of these DNA preparations were compared with genomic DNA isolated by a standard kit (Flexi gene DNA kit), using amplification of exon 2 of CYP19 (aromatase) gene. The similar and significant amplification of this gene was obtained using genomic DNA isolated by kit and various detergents. However, among the detergents used, the Rin and Ezee were found to be the best to get DNA of high purity comparable to that obtained by kit.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
DNA
/
Cabras
/
Aromatase
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Detergentes
/
Eletroforese em Gel de Ágar
/
Animais
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2006
Tipo de documento:
Artigo
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