Isolation of etiological agent of hydropericardium syndrome in chicken embryo liver cell culture and its serological characterization.
Indian J Exp Biol
;
2003 Aug; 41(8): 821-6
Artigo
em Inglês
| IMSEAR
| ID: sea-56364
ABSTRACT
The virus causing hydropericardium syndrome was isolated in chicken embryo liver (CEL) cell culture from livers obtained from naturally infected broilers. The cytopathic effects characterized by rounding and degeneration of cells were visible 36 hr post infection in first passage. At 4th passage level, the infectivity titre was 5.24 log10 TCID50/ml. In May-Grunwald and Giemsa stained cells, basophilic intranuclear inclusions ('bird eye' inclusion), typical of aviadenovirus infection, were observed. The specificity of inclusion was confirmed by indirect immunofluorescence. Various serological tests, such as agar gel precipitation test, counter immuno electrophoresis, micro serum neutralization test and enzyme linked immunosorbent assay were also standardized to confirm the isolation of etiological agent of hydropericardium syndrome in CEL cell culture and to diagnose the disease in poultry.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Derrame Pericárdico
/
Doenças das Aves Domésticas
/
Testes Sorológicos
/
Embrião de Galinha
/
Infecções por Adenoviridae
/
Aviadenovirus
/
Efeito Citopatogênico Viral
/
Corpos de Inclusão Viral
/
Animais
Tipo de estudo:
Estudo diagnóstico
/
Estudo de etiologia
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2003
Tipo de documento:
Artigo
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