A dot-immunobinding assay (dot-Iba) for rapid diagnosis of pulmonary tuberculosis.
Indian J Exp Biol
;
2001 Oct; 39(10): 984-8
Artigo
em Inglês
| IMSEAR
| ID: sea-56592
ABSTRACT
IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Escarro
/
Tuberculose Pulmonar
/
Idoso
/
Feminino
/
Humanos
/
Masculino
/
Imunoensaio
/
Immunoblotting
/
Criança
/
Pré-Escolar
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2001
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS