Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain.
Indian J Exp Biol
;
1994 May; 32(5): 345-7
Artigo
em Inglês
| IMSEAR
| ID: sea-56832
ABSTRACT
RNA isolation from purified cucumber mosaic virus, CMV-U strain and cDNA synthesis was carried out. The coat protein gene (RNA4) region was amplified selectively by polymerase chain reaction (PCR) with CMV RNA4-specific primers. Double-stranded cDNA was cloned in PRT103 vector at Xho1/Kpn1 site and about 1kb insert obtained. The insert was partly sequenced which showed 50% sequence homology with CMV-Q, C and WL strains.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Dados de Sequência Molecular
/
Sequência de Bases
/
Capsídeo
/
Clonagem Molecular
/
Cucumovirus
/
Genes Virais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1994
Tipo de documento:
Artigo
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