Enhancement of haemopoietic stem cell adherence to bone-marrow derived stroma by phyto-haemagglutinin treated leucocyte conditioned medium (PHA-LCM).
Indian J Exp Biol
;
1994 Apr; 32(4): 225-8
Artigo
em Inglês
| IMSEAR
| ID: sea-56984
ABSTRACT
Stem cell adhesion to bone-marrow derived stroma, plays a crucial role in haemopoiesis. However, there is very little information as to the nature of the adhesion molecule. In this paper we have shown that human bone-marrow derived stroma can be established in tissue culture. This stroma is able to adhere human bone-marrow mononuclear cells including the multipotent stem cell, viz. CFU-GEMM. Their adherence increases when the stroma is treated with lymphokines in the form of PHA-treated leucocyte conditioned medium (PHA-LCM). Triton X-100 extracts of the untreated and PHA-LCM treated stroma were analysed on single dimension PAGE. It was observed that PHA-LCM treated stromal extracts showed two extra bands and an increase in the density of a band of approximately 14 kDa. Whether these changes have anything to do with the increased adhesion of stem cell is not yet known.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fito-Hemaglutininas
/
Medula Óssea
/
Humanos
/
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
/
Adesão Celular
/
Células Cultivadas
/
Células Estromais
/
Meios de Cultivo Condicionados
/
Leucócitos
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1994
Tipo de documento:
Artigo
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