Construction of a gene bank of Rhizobium leguminosarum Rld 164.
Indian J Exp Biol
;
1989 Mar; 27(3): 288-9
Artigo
em Inglês
| IMSEAR
| ID: sea-57134
ABSTRACT
The total genomic DNA of R. leguminosarum Rld164 (trp, sms, azi) was cloned in the EcoR1 site of the wide host and conjugally transferable cosmid vector pLAFR1. The average insert size in the gene clones of the bank was found to be 21.3 Kb. The strain R. leguminosarum Rld7 (leu-1) was employed as recepient to conjugally transfer and thus isolate the complementary leu+ allele carrying clones from the gene bank.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Rhizobium
/
DNA Bacteriano
/
Biblioteca Gênica
/
Clonagem Molecular
/
Genes Bacterianos
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1989
Tipo de documento:
Artigo
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