Purification and characterization of phospholipase C of Salmonella gallinarum.
Indian J Exp Biol
;
1998 Dec; 36(12): 1245-52
Artigo
em Inglês
| IMSEAR
| ID: sea-57151
ABSTRACT
Phospholipase C was isolated from an outbreak strain of Salmonella gallinarum with ciprofloxacin extraction, dialysis, gel filtration, ion exchange chromatography and chromatofocussing. Purified phospholipase C (mol wt. 65 KDa; isoelectric point, pI 3.5) was resistant to pasteurization, stomach enzyme (pepsin), bacterial protease and lipase but lost its activity on trypsin and chymotrypsin treatment. It was sensitive to pH > or = 8.0. It was haemolytic, embryotoxic, enterohaemorrhagic, lethal to birds, cytotoxic to Vero and MDBK cells, dermonecrotoxic in rabbit and antigenically active protein. Antisera raised against purified phospholipase C neutralized its all biological activities and agglutinated the producer Salmonella strains. Serologically it was proved similar to phospholipase C of Klebsiella pneumoniae and S. weltevreden. Fluorescent antibody technique (FAT) was standardized to detect phospholipase producer strains.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fosfolipases Tipo C
/
Salmonella
/
Linhagem Celular
/
Sobrevivência Celular
/
Cromatografia em Gel
/
Eletroforese em Gel de Poliacrilamida
/
Dose Letal Mediana
/
Animais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1998
Tipo de documento:
Artigo
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