Phage-mediated conjugation in staphylococci as a means of transfer of drug-resistance.
Indian J Exp Biol
;
1995 Jul; 33(7): 505-8
Artigo
em Inglês
| IMSEAR
| ID: sea-57296
ABSTRACT
Penicillin resistance plasmid was transferred from Staphylococcus aureus B4 (PcrKms, donor) to S. aureus ML351 (PcsKmr, recipient) by co-cultivation of the donor with the recipient in nutrient broth with or without the modifying effects of CaCl2 or sodium dodecyl sulfate. It was found that the transfer of drug-resistance occurred maximally between 6 and 18 hr postinoculation; however, addition of DNase (200 micrograms/ml) could totally prevent such a transfer up to 6 hr and significantly reduce it thereafter. Cell-free filtrate of the donor culture when mixed with the recipient was ineffective in bringing about the transfer of Pcr.
Texto completo:
DisponíveL
Índice:
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Assunto principal:
Plasmídeos
/
Staphylococcus aureus
/
Resistência às Penicilinas
/
Resistência a Canamicina
/
Fagos de Staphylococcus
/
Técnicas de Transferência de Genes
/
Conjugação Genética
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1995
Tipo de documento:
Artigo
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