Production and characterisation of a monoclonal antibody to progesterone and its effect on fertility.
Indian J Exp Biol
;
1991 Jan; 29(1): 6-11
Artigo
em Inglês
| IMSEAR
| ID: sea-57481
ABSTRACT
A monoclonal antibody reacting with progesterone has been raised by fusion of mouse myeloma cells (SP20) and splenocytes of BALB/c mice hyperimmunized with 4-pregnane 3,20 dione conjugated to bovine serum albumin. Association constant of this antibody for binding with progesterone was 0.22 x 10(9) l/mole. The antibody was highly specific for progesterone. A single ip injection of this antibody brought about an antifertility effect which is influenced by genotype. Antibody treatment brought about a significant decrease in the fetal weight and a slight decrease in the plasma progesterone levels. The antifertility effect could be reversed only up to day 3 by exogenous administration of progesterone.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Progesterona
/
Feminino
/
Masculino
/
Fertilidade
/
Animais
/
Camundongos
/
Camundongos Endogâmicos BALB C
/
Anticorpos Monoclonais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1991
Tipo de documento:
Artigo
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