Distinguishing clonal apple rootstocks by isozymes banding patterns.
Indian J Exp Biol
;
2001 Nov; 39(11): 1149-55
Artigo
em Inglês
| IMSEAR
| ID: sea-57519
ABSTRACT
Molecular characterisation of clonal apple rootstocks using isozymes was carried out to identify isozyme polymorphism in seven clonal apple rootstocks and to identify the most characteristic and stable enzyme markers for each individual rootstock. Five enzyme systems were studied out of which polyphenol oxidase, malate dehydrogenase, acid phosphatase and peroxidase were useful in discriminating among the rootstocks. The peroxidase enzyme system showed maximum variation and esterase showed the least variation among the rootstocks. Out of seven rootstocks, three were distinguished on the basis of one enzyme system only (M.3 with MDH or PER, M.7 with PPO or PER and MM. 111 with MDH). Out of the sixteen loci studied seven were found to be polymorphic. Genetic variation among the rootstocks was explained on the basis of various parameters. The percentage of polymorphic loci varied from 13.33 to 35.71 per cent.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Peroxidases
/
Polimorfismo Genético
/
Fosfatase Ácida
/
Variação Genética
/
Catecol Oxidase
/
Malus
/
Esterases
/
Isoenzimas
/
Malato Desidrogenase
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2001
Tipo de documento:
Artigo
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