Isolation and purification of a lethal scorpion toxin with neuromuscular blocking activity.
Indian J Exp Biol
;
1990 May; 28(5): 451-4
Artigo
em Inglês
| IMSEAR
| ID: sea-57851
ABSTRACT
Toxin-L a lethal neuromuscular blocking agent was isolated from the venom of the scorpion, Lychas laevifrons (Pocock), by the CM-cellulose ion-exchange chromatography. It was a homogenous, thermolabile and low molecular weight protein. The toxin produced irreversible blockade of indirect stimulation induced twitch responses on innervated rat phrenic nerve-diaphragm and chick biventer cervicis preparation. The toxin did not produce any contractile response on toad rectus abdominis muscle preparation. On chronically denervated rat diaphragm, the toxin failed to alter the responses induced by direct stimulation, exogenous acetylcholine, potassium chloride and caffeine. Acetylcholine and carbachol induced contractions on isolated chick biventer cervicis remained unaltered by the toxin. Neostigmine failed to alter toxin induced neuromuscular blockade on innervated rat diaphragm. The toxin released a significant amount of acetylcholine from innervated rat diaphragm. It may be concluded that the toxin acts presynaptically through the release of acetylcholine, thereby producing neuromuscular blockade.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Venenos de Escorpião
/
Animais
/
Peso Molecular
/
Bloqueadores Neuromusculares
/
Junção Neuromuscular
/
Neurotoxinas
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1990
Tipo de documento:
Artigo
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