Cytokinin oxidase activity and cytokinin content in roots of sunflower under water stress.
Indian J Exp Biol
;
2001 Aug; 39(8): 786-92
Artigo
em Inglês
| IMSEAR
| ID: sea-58031
ABSTRACT
Contents of trans-zeatin riboside (ZR), dihydrozeatin riboside (DZR) and N6-(delta2-isopentenyl) adenosine (iPA) was quantified by an indirect ELISA using polyclonal antibodies, in the roots, xylem sap and leaves of pot grown sunflower plants subjected to water stress (RWC of leaves approximately 65 per cent). The delivery rates of all three cytokinins decreased significantly under stress. Cytokinin levels also decreased in roots and in leaves of stressed plants. Three-fold increase in cytokinin oxidase activity was observed in stressed roots after polymin P-ammonium sulphate fractionation. Further purification using Con A agarose resulted in elution of protein with cytokinin oxidase activity and was found to be 30 kDa protein on SDS-PAGE.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oxirredutases
/
Ensaio de Imunoadsorção Enzimática
/
Água
/
Cromatografia de Afinidade
/
Cromatografia Líquida de Alta Pressão
/
Citocininas
/
Eletroforese em Gel de Poliacrilamida
/
Helianthus
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2001
Tipo de documento:
Artigo
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