New Helicoverpa armigera Hbn cell line from larval hemocyte for baculovirus studies.
Indian J Exp Biol
;
2002 Jan; 40(1): 69-73
Artigo
em Inglês
| IMSEAR
| ID: sea-58483
ABSTRACT
A new cell line from the larval hemocytes of H. armigera was established in Grace's medium modified by adding lactalbumin hydrolysate and yeastolate (3.3g/l), and supplemented with fetal bovine serum (20%). The cell line was designated as NIV-HA-1195. The cell population at P-78 consisted mainly of epithelial-like cells (89.36%), fibroblast-like cells (8.31%) and giant cells (2.13%). The population doubling time was 96hr at P-8, 60hr at P-43. The chromosome number ranged from 45 to 200. The cell line is susceptible to the baculoviruses, Autographa californica nucleopolyhedrovirus (AcNPV), Spodoptera litura NPV and the homologous HaNPV. Isoenzyme profile and results of 16S rRNA heteroduplex analysis clearly indicated the species specificity of the new cell line.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Divisão Celular
/
Linhagem Celular
/
Baculoviridae
/
Análise Heteroduplex
/
Glucosefosfato Desidrogenase
/
Isocitrato Desidrogenase
/
L-Lactato Desidrogenase
/
Larva
/
Animais
/
Malato Desidrogenase
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2002
Tipo de documento:
Artigo
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