Partial characterization of serum immunoglobulins of Oreochromis mossambicus.
Indian J Exp Biol
;
2000 Jun; 38(6): 549-53
Artigo
em Inglês
| IMSEAR
| ID: sea-58497
ABSTRACT
Serum immunoglobulins of O. mossambicus were purified using chromatography methods--CM affinity gel blue chromatography followed by two step purification involving a combination of ion-exchange and gel filtration chromatography. Studies revealed that O. mossambicus produces only one class of high molecular weight macroglobulin as determined by molecular sieving by Sepharose CL 6-B. Immunoelectrophoresis of purified O. mossambicus serum against rabbit anti O. mossambicus serum gave only a single precipitin line. Further analysis of the immunoglobulin by SDS-PAGE showed that the IgM macroglobulin weighs about 900,000 Da, composed of mu-like heavy chain weighing about 90 kDa each and light chains weighing about 30 kDa each.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Precipitinas
/
Coelhos
/
Eletroforese das Proteínas Sanguíneas
/
Imunoglobulina M
/
Imunoglobulinas
/
Soroalbumina Bovina
/
Bovinos
/
Testes de Hemaglutinação
/
Imunoeletroforese
/
Cromatografia em Gel
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2000
Tipo de documento:
Artigo
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